Science Behind Our Products

Liposomes are spherical vesicles that are non-toxic, non-immunogenic and biodegradable. They can encapsulate both hydrophobic and hydrophilic active ingredients and deliver them more effectively into the body.

LipoQuest™ Products

Vesta Nutra manufactures a variety of products with enhanced delivery systems using liposomal technology. The expanding list of active ingredients incorporated in liposomes include:

Vitamin C	Glutathione
Vitamin B₁₂	Pearl Tomato®
Vitamin D₃	Hyal-Flex™ Hyaluronic Acid
	CoQ10

Useful Terms That Help Understand

The Science Behind Our Products

Enhancing product quality and efficiency across industries.

Quantification of Loading

Active ingredients are encapsulated inside liposomal material. The loading amount plays a pivotal role in the bioavailability of the product. One way to quantify the amount of loading is by measuring the percent encapsulation of the active ingredient in the liposome. Higher loading is correlated with longer shelf-life and better stability of the nanocarrier.

Particle Stability

Zeta Potential measures surface charge and is an index of particle stability. The larger the Zeta Potential absolute value, the larger the amount of surface charge.

Liposome Confirmation

Characterization of liposomes is completed by Cryogenic Transmission Electron Microscopy (Cryo-TEM). Successful images will clearly show a uniform round particle size. Cryo-TEM is routinely used to visualize amphiphilic structures in aqueous solutions.

Particle Size

Dynamic Light Scattering (DLS) is a non-invasive spectroscopic method that can determine the distribution of particles, including liposomes, in solution or suspension. DLS can be used to verify a uniform size and size distribution lower than 1nm. Liposomes are typically around 100nm.

Morphology and Particle Size Determination

Transmission electron microscopy (TEM) can be used to establish the size and shape of liposomes. Particle size of powders can be studied by scanning electron microscopy (SEM). The size distribution and surface morphology can be observed.

Encapsulation Efficiency

The amount of active ingredient represented by the percentage of active ingredient inside the liposome when compared with the total amount of active ingredient present. It can be quantified by a variety of methods that include size exclusion chromatography, or by ultrafiltration-centrifugation techniques.

In the Ultrafiltration-centrifugation technique, the free active ingredient can be separated from liposomes by spinning a solution in an ultracentrifuge. After spinning the solution, the liposomes form a solid pellet and can be isolated from the filtrate that contains the unbound material. Using spectroscopic methods, like HPLC, LCMS and absorbance readers, the concentration of the unbound material can be quantitated.

Encapsulation efficiency (EE%) can be calculated by the difference between the total (Ctotal) and free active ingredient (Cf) concentrations.

EE%= [Ctotal-Cf)/Ctotal] x 100

Unpublished data

LipoQuest™ CoQ10 has enhanced in vitro cell uptake. Studies using HEK 293 cells show a ~3X increase in cell permeability compared to CoQ10. In addition, these studies also documented a 6-fold increase in solubility in aqueous environments compared to CoQ10.This result has been shown for other lipophilic active ingredients.
Similar experiments with LipoQuest™ Vitamin C are in progress.
LipoQuest™ Vitamin C has been visualized using cryo EM imaging. Spherical liposomes have been documented with an average radius of ~30nm-50nm.
Encapsulation Efficiencies are currently being assessed for LipoQuest™ Vitamin C using both ultracentrifuge method. Analysis by LCMS, and also by Zeta Potential.

Our Qualifications

We Guarantee Our Quality!!

Halal-certified products are those which have apparently met the requirements of Islamic law and are suitable for the consumption of Muslims. Halal is an Arabic word which means permissible — as opposed to ‘haram’.

A Halal certification is a guarantee that the food is prepared adhering to the Islamic law and is unadulterated.

Hazard analysis and critical control points, or HACCP, is a systematic preventive approach to food safety from biological, chemical, and physical hazards in production processes that can cause the finished product to be unsafe and designs measures to reduce these risks to a safe level.

A HACCP certification serves as proof of your food establishment's commitment to upholding food safety regulations.

The Food and Drug Administration (FDA) is responsible for protecting the public health by assuring the safety, efficacy, and security of human and veterinary drugs, biological products, medical devices, our nation’s food supply, cosmetics, and products that emit radiation.

FDA fulfills this responsibility by ensuring the security of the food supply and by fostering development of medical products to respond to deliberate and naturally emerging public health threats.

cGMP refers to the Current Good Manufacturing Practice regulations enforced by the FDA. CGMP provides for systems that assure proper design, monitoring, and control of manufacturing processes and facilities.

CGMP requirements help to assure the safety and efficacy of drug products.

A National Sanitation Foundation (NSF) certification is a certification that signifies a food product meets the public health and safety standards set by the NSF

The certification process entails a constant evaluation and inspection of equipment, facilities and production procedures and their ability to meet the safety standards

The Natural Products Association is an all-inclusive trade association that serves retailers, suppliers, manufacturers, distributors, brokers, consultants and other entities that support the natural products industry.

the largest and oldest nonprofit organization representing the interests of manufacturers and retailers of the natural products industry,

Non-GMO means a product was produced without genetic engineering and its ingredients are not derived from GMOs. Non-GMO Project Verified additionally means that a product is compliant with the Non-GMO Project Standard, which includes stringent provisions for testing, traceability, and segregation.

Non-GMO certified products have been verified to have been grown and processed without genetic modification.

This certification verifies that the ingredients, production process including all machinery, and/or food-service process complies with the standards of kashrut (Jewish dietary law) as stipulated in the Shulchan Arukh, the benchmark of religious Jewish law.

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All ingredients and sub-ingredients used in a product must be kosher.

Get In Touch With Us

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Email

marketing@vestanutra.com

Phone

888-558-3782